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Product Categories
Tag antibody
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Product Name
Flag Tag (10E1) Mouse mAb
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Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
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Alternative Names
DYKDDDDK Tag; Flag; FLAG
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Immunogen
Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Dilution Ratio
WB: 1/1000-1/10000 IP: 1/500
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Molecular Weight
Refer to figures
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Clonality
Monoclonal Antibody
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Gene Name
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Conjugation
Unconjugated
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Isotype
IgG2b
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Host
Mouse
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Application
WB,IP
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Purification
Affinity Purified
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Reactivity
Species-independent
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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Form
Liquid
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Clonality No.
10E1-1D8-5F5
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SwissProt ID
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Research Field
Tags & Cell Markers
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Gene ID
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Modification
Unmodified