Loading Control Mouse mAb Kit (HRP) - Chengdu Zhengneng Biotechnology Co., Ltd

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Cat.#： 200300

Alternative Names： ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta; Beta 4 tubulin; TBB5; TUBB2; TUBB2A; tubulin beta 2A; beta tubulin; tubulin beta chain; G3PD; GAPD; GAPDH; HEL-S-162eP

Application： WB

Reactivity： Human,Mouse,Rat

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Loading control antibody
Product Name

Loading Control Mouse mAb Kit (HRP)
Background

This kit is consist of three different but reliable antibodies which are specific to the most popular three loading control targets: GAPDH, β-Actin and β-Tubulin, also with three individual package. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells(Swiss-Prot Acc.P60709). Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (Swiss-Prot Acc.P07437). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer diseases (Swiss-Prot Acc.P04406).
Alternative Names

ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta; Beta 4 tubulin; TBB5; TUBB2; TUBB2A; tubulin beta 2A; beta tubulin; tubulin beta chain; G3PD; GAPD; GAPDH; HEL-S-162eP
Immunogen

-
Buffer System

Beta actin: In PBS(pH 7.4) containing with 0.1mg/ml BSA and 50% glycerol. Beta Tubulin: In PBS(pH 7.4) containing with 0.1mg/ml BSA and 50% glycerol. GAPDH: In PBS(pH 7.4) containing with 50% glycerol.
Dilution Ratio

WB: 1/10000-1/20000
Molecular Weight

Beta actin: 42 kDa; Beta Tubulin: 55 kDa; GAPDH: 36 kDa
Clonality

Monoclonal antibody
Gene Name

ACTB/TUBB/GAPDH
Conjugation

HRP
Isotype

Beta actin: IgG2b; Beta Tubulin: IgG1; GAPDH: IgM
Host

Mouse
Application

WB
Purification

Affinity purified
Reactivity

Human,Mouse,Rat
Storage

Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Form

liquid
Clonality No.

-
SwissProt ID

P60709/P07437/P04406
Research Field

-
Gene ID

60/203068/2597
Modification

Unmodified