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Cat.#:
250174
Alternative Names:
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
Application:
WB
Reactivity:
Human,Rat,Mouse,Monkey,Dog
Price:
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Loading Control antibody
Product Name
beta Actin (5C1) Mouse mAb
Background
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Alternative Names
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
Immunogen
Synthetic Peptide of β-actin
Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Dilution Ratio
WB: 1/5000-1/10000
Molecular Weight
Calculated MW: 42 kDa; Observed MW: 42 kDa
Clonality
Monoclonal Antibody
Gene Name
ACTB
Conjugation
Unconjugated
Isotype
IgG1
Host
Mouse
Application
WB
Purification
Affinity Purified
Reactivity
Human,Rat,Mouse,Monkey,Dog
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Form
Liquid
Clonality No.
5C1-4B9-8C7
SwissProt ID
P60709
Research Field
Cell Biology
Gene ID
60
Modification
Unmodified
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