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Product Categories
Primary antibody
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Product Name
BRCA1 Rabbit pAb
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Background
E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).
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Alternative Names
BRCA1; RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53
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Immunogen
The antiserum was produced against synthesized peptide derived from human BRCA1.
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
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Molecular Weight
Calculated MW: 208 kDa; Observed MW: 208 kDa
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Clonality
Polyclonal Antibody
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Gene Name
BRCA1
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Conjugation
Unconjugated
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Isotype
IgG
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Host
Rabbit
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Application
WB,IHC-F,IHC-P,ICC/IF,ELISA
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Purification
Affinity Purified
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Reactivity
Human,Rat
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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Form
Liquid
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Clonality No.
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SwissProt ID
P38398
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Research Field
Epigenetics and Nuclear Signaling
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Gene ID
672
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Modification
Unmodified