-
Product Categories
Primary antibody
-
Product Name
AKT Mouse mAb
-
Background
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
-
Alternative Names
AKT1
-
Immunogen
Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.
-
Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
-
Dilution Ratio
WB: 1/500-1/1000 IP: 1/20
-
Molecular Weight
Calculated MW: 56 kDa; Observed MW: 60 kDa
-
Clonality
Monoclonal Antibody
-
Gene Name
AKT1/AKT2/AKT3
-
Conjugation
Unconjugated
-
Isotype
IgG1
-
Host
Mouse
-
Application
WB,IP
-
Purification
Affinity Purified
-
Reactivity
Human,Mouse,Rat
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
-
Form
Liquid
-
Clonality No.
3B11-G8-B1
-
SwissProt ID
P31749/P31751/Q9Y243
-
Research Field
Signal Transduction
-
Gene ID
207/208/10000
-
Modification
Unmodified